Structural stability of bacteriorhodopsin solubilized by n-nonyl-beta-glucoside

Functional bacteriorhodopsin is efficiently solubilized and delivered to membranes by the chaperonin GroEL.

Soluble complexes between the tetradecameric chaperonin GroEL and integral membrane proteins can be efficiently formed by detergent dialysis. For example, GroEL14 was found to bind a limit of two molecules of bacteriorhodopsin (BR). The GroEL-solubilized BR molecules were rapidly ejected from the chaperonin complexes on the addition of ATP or adenosine 5'-[beta,gamma-imido]triphosphate but not ...

Density Functional Study on Stability and Structural Properties of Cu n clusters

In this research DFT/B3LYP method has been employed to investigate the geometrical structures,relative stabilities, and electronic properties of Cun (n=3–10) clusters for clarifying the effect of sizeon the properties. Through a careful analysis of the successive binding energies, second-orderdifference of energy and the highest occupied-lowest unoccupied molecular orbital energy gaps as afunct...

Stability of limonin glucoside in beverage matrices

BACKGROUND: We completed a study over a 200-day period examining the stability of limonin glucoside formulated into three beverage matrices. RESULTS: Beverages containing limonin glucoside were found to contain limonin (0.13–20.10 mg L−1) during their initial testing; however, these concentrations were directly attributable to the presence of contaminating limonin in the particular lot of limon...

Structural changes in bacteriorhodopsin induced by electric impulses

Electric impulses of high field intensity (2 x 105 to 3 × 106 Vm1, 1 to 20 #s duration) cause transient changes in the optical absorbance of suspended purple membranes ofHalobacterium halobium. The electric dichroism at 1 mM NaCl, pH ~ 6,and at 293K is dependent on field strength, pulse duration and wavelength of the monitoring, plane-polarized light in the range 400 to 650 nm. The optically de...

Heteronuclear multidimensional NMR spectroscopy of solubilized membrane proteins: resonance assignment of native bacteriorhodopsin.